<a href='https://brc.riken.jp/mus/pcr06410'>Genotyping protocol -PCR-</a> Developed by Tatsushi Muta, Graduate School of Life Sciences, Tohoku University. Bruce 4 ES cells were used to generate the mutant mice. A frt-neo cassette was removed by crossing with CAG-FLPe (B6) mice. Cre/loxP system B6.Cg-Nfkbiz<tm1.1Muta> B6.Cg-Nfkbiz<tm1.1Muta> Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> IκB-ζ (Nfkbiz) 遺伝子のfloxedマウス。エクソン5から7がloxPで挟まれている。 Bruce 4 [B6.Cg-Thy1<a>] Nfkbiz gene floxed mice. Exons 5 to 7 were flanked by loxP sites. Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] <A HREF="https://mus.brc.riken.jp/ja/mouse_of_month/apr_2017_mm" target="_blank">Mouse of the Month Apr 2017</A> C (3-6 months) 東北大学大学院生命科学研究科・牟田達史先生。Bruce 4 ES細胞を用いて作出。Frt-neoカセットはCAG-FLPe（B6）マウスとの交配より除去済。 C（3〜6か月） 丸山　貴司 Maruyama, Takashi IκB-ζ flox/flox, B6-Nfkbiz floxed IκB-ζ flox/flox, B6-Nfkbiz floxed P1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Nfkbiz genomic DNA RBRC06410 FLP/frt system 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。 true